Table 1 Primers and siRNA sequences
From: MicroRNA let-7b inhibits cell proliferation via upregulation of p21 in hepatocellular carcinoma
Name | Primer sequence |
|---|---|
β-actin F | 5′-CAGCAAGCAGGAGTATGACG-3′ |
β-actin R | 5′-GAAAGGGTGTAACGCAACTAA-3′ |
U6 F | 5′-GTGCTCGCTTCGGCAGCACATATAC-3′ |
U6 R | 5′-AAAAATATGGAACGCTCACGAATTTG-3′ |
p21 F | 5′-AAACTAGGCGGTTGAATGAG-3′ |
p21 R | 5′-AAAGGAGAACACGGGATGAG-3′ |
P21 siRNA (sense) | 5′-GACCAUGUGGACCUGUCACdTdT-3′ |
P21 siRNA (antisense) | 5′-GUGACAGGUCCACAUGGUCdTdT-3′ |
NC siRNA (sense) | 5′-AAUUCUCCGAACGUGUCACdTdT-3′ |
NC siRNA (antisense) | 5′-GUGACACGUUCGGAGAAUUdTdT-3′ |
Let-7b mimics | 5′-UGAGGUAGUAGGUUGUGUGGUU-3′ |
NC mimics | 5′-UUGUACUACACAAAAGUACUG-3′ |
Let-7b F | 5′-GTACTGAGGTAGTAGGTTGT-3′ |
Let-7b R | 5′-GTGCAGGGTCCGAGGT-3′ |
Anti-Let-7b | 5′-ACACGAATTCAACCACACAACCTACTACCTCATATACAACCACACAACCTACTACCTCAACATCAACCACACAACCTACTACCTCATCTTCAAACCACACAACCTACTACCTCAGGATCCACAC-3′ |