Fig. 5
From: MicroRNA let-7b inhibits cell proliferation via upregulation of p21 in hepatocellular carcinoma
Let-7b suppress HCC cell proliferation by upregulating P21. a HepG2 and QGY-7703 cell lines were transfected with p21 siRNA, and p21 mRNA levels were examined by RT-PCR. Data from three replicates are shown as the means (± SD). **P < 0.01. b HepG2 and QGY-7703 cell lines were transfected with p21 siRNA, and p21 protein levels were examined by western blot. c, d CCK-8 assay was used to detect cell proliferation of HepG2 and QGY-7703 cell lines treated with p21 siRNA. Data from three replicates are shown as the means (± SD). **P < 0.01. e, f Flow cytometric analysis was performed to test the cell cyclesof HepG2 and QGY-7703 cell lines treated with p21 siRNA. Data from three replicates are shown as the means (± SD). *P < 0.05