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Fig. 5 | Cell & Bioscience

Fig. 5

From: KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis

Fig. 5

KLF13 reduces cholesterol biosynthesis and transcriptionally represses HMGCS1. a Cholesterol content was detected in shCtrl, shKLF13#1 and shKLF13#2 HCT116 and HT-29 cells. *p < 0.05. b Cholesterol content was detected in Ctrl and KLF13 overexpressed HCT116 and HT-29 cells. *p < 0.05. c shCtrl and shKLF13#1 HCT116 or HT-29 cells were subjected to RT-qPCR and Western blot analysis of HMGCS. **p < 0.01. d Ctrl and KLF13 overexpressed HCT116 or HT-29 cells were subjected to RT-qPCR and Western blot analysis of HMGCS. **p < 0.01. e Luciferase activity of HMGCS1 promoter was measured in siCtrl, siKLF13, Ctrl and KLF13 overexpressed HCT116 and HT-29 cells. **p < 0.01. f ChIP-qPCR assay was performed in KLF13 knockdown HCT116 and HT-29 cells to determine KLF13 binding to HMGCS1 promoter. **p < 0.01

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