Fig. 1

Expression of XIAP in response to XIAP silencing in MCF-7 cells. a Fold-change expression of XIAP gene. The expression level of XIAP mRNA was reduced in siRNA treated cells compared to the si-NEG after 24, 48 and 72 h post-transfection. ** illustrates the P < 0.001; *** represents P < 0.0001. b Protein expression level of XIAP in response to XIAP siRNA. Western blot shows that the highest down-regulation of protein occurred in 48 h post-transfection. c Densitometry of XIAP protein bands. The results of western blot were quantified by ImageJ software in order to measure the density of protein bands. The si-NEG was employed as a control group which exhibits the normal expression quantity of XIAP. The densitometry analysis revealed the low amount of XIAP protein at 48 h after transfection