Fig. 5

Metoprolol improved the AVP-induced decrease in the protein expression levels of AKT1 and SERCA2. a Left panel, F-actin staining (scale bar = 200 μm) was performed to measure the hypertrophic growth of the H9C2 cells treated with AVP, Met, and Met + AVP. Right panel, Quantification of the surface area of the H9C2 cells induced with AVP, Met, and Met + AVP. b The mRNA levels of ANP, BNP and β-MHC were measured in H9C2 cells treated with AVP, Met, and Met + AVP. c, d The protein expression and quantification of the P-AKT1(Thr308), AKT1, SERCA2 and PLN levels in the AVP-, Met-, and Met + AVP-treated H9C2 cells, GAPDH was selected as the loading control. e, f Fluo-3/AM was used to measure intracellular calcium concentration. The fluorescence (scale bar = 100 μm) and quantification of the calcium concentration in the AVP-, Met-, and Met + AVP-induced H9C2 cells. All the data are presented as the mean ± S.E.M. of at least three independent experiments. ^*,#,§Both indicates P < 0.05; *compared with Control; ^#compared with AVP; ^§compared with Met