Fig. 4
AKT1 overexpression upregulated the protein expression of SERCA2 and downregulated the protein expression of PLN. a, b The protein expression and quantification of the SERCA2 and PLN in the AKT1 overexpressing stable strain. GAPDH was used as the loading control. c, d Fluo-3/AM was used to measure the intracellular calcium concentration. The fluorescence (scale bar = 100 μm) and quantification of the calcium concentration in the cells. All the data are presented as the mean ± S.E.M. of at least three independent experiments. *, #,ΔBoth indicates P < 0.05. *Compared with Control; #compared with Control + AVP; Δcompared with LV-AKT1