Fig. 3

AKT1 overexpression inhibited the AVP-induced H9C2 hypertrophy. a, b The protein expression and quantification of P-AKT1(Thr308), AKT1 and AKT2 levels in the AKT1 overexpressing H9C2 stable strain, GAPDH was taken as the internal control. c, d α-Actinin staining (scale bar = 20 μm) was performed to determine the hypertrophic levels of the Control and AKT1 overexpressing H9C2 cells treated with AVP. e The mRNA levels of ANP, BNP and β-MHC were measured in the AKT1 overexpressing H9C2 stable strain. All the data are presented as the mean ± S.E.M. of at least three independent experiments. *^,#,ΔP < 0.05; NS not significant. *Compared with Con; ^#compared with Con + AVP; ^Δcompared with LV-AKT1