Fig. 4

SHP1 promotes NO production through JAK1/STAT3 signals, and me^v/me^v MSCs alleviated ConA-induced liver injury more obviously compared to WT MSCs. WT and me^v/me^v MSCs were treated with IFNγ and TNFα (20 ng/ml) for the indicated times. a The phosphorylated JAK1 and STAT3 as well as total JAK1 and STAT3 were evaluated by WB. b The gray intensity of pJAK1 and pSTAT3 was calculated by ImageJ software. c The phosphorylated P38, JNK, P65 and AKT were determined by WB. d The gray intensity of PP38, PP65 and PAKT was calculated by ImageJ software. ConA (15 mg/kg) was intravenously injected with WT or me^v/me^v MSCs, and the serum and liver samples were isolated 8 h later. e The liver sections were stained by hematoxylin & eosin. Scale bar: 50 μm. f The area of necrosis in liver was calculated by ImageJ software. g The levels of ALT and AST in serum were measured. The experiments were repeated at least three times. Error bars represent ± SEM (n = 5), ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001