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Fig. 3 | Cell & Bioscience

Fig. 3

From: Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals

Fig. 3

SHP1 regulated NO production under inflammatory cytokines stimulation. mev/mev MSCs and WT MSCs were treated with IFNγ, TNFα or together (20 ng/ml) for 24 h. a The NO content in supernatant was assayed as total nitrate by using modified Griess reagent. The inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (Cox2) mRNA in mev/mev MSCs and WT MSCs were analyzed by real-time PCR. b The protein level of iNOS and Cox2 in mev/mev MSCs and WT MSCs were determined by western blotting (WB). c WT MSCs were transfected by lentivirus containing an SHP1-expression vector or control vector, and SHP1 expression was measured by WB. d The NO content in supernatant was assayed as total nitrate by using modified Griess reagent. e The mRNA level of iNOS and Cox2 were analyzed by real-time PCR. f The protein level of iNOS and Cox2 were analyzed by WB. The experiments were repeated at least three times. Error bars represent ± SEM (n = 3), ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001

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