Fig. 5

Peak levels of TR binding at the climax of intestinal metamorphosis correlates with peak levels of RNA polymerase II recruitment and H3K79 methylation in the intestine during natural metamorphosis. ChIP assays were performed with indicated antibodies on the intestine of tadpoles at different metamorphic stages. a TR binding to the TRE region of Mettl1 gene at metamorphic climax stages (stage 60 and 62) is increased compared to that at premetamorphic stage 54. After climax, TR binding is reduced in TRE region of Mettl1 gene. b The level of dimethylated H3K79 in the TRE region of Mettl1 gene peaks in the intestine at metamorphic climax. c RNA polymerase II recruitment to the TRE region of Mettl1 promoter also peaks at metamorphic climax period. d Only background ChIP signal is detected with the negative control IgG ChIP throughout metamorphosis. n.s. not significant