Fig. 6

BMP2K overexpression confers AMKL cells of resistance to multiple chemotherapy compounds. Control and BMP2K overexpression CMK cells were cultured with Vehicle or chemotherapy compounds (10 ng/ml Nocodazole, 2.5 nM Methotrexate, 50 nM TPA and 10 μM Etoposide) for 3 days. The cell numbers were counted on each day for cell proliferation assay a Apoptosis was measured on day 1 by Annexin V and 7-AAD staining and analyzed by flow cytometry b The cell cycle profile was also measured by PI staining and analyzed by flow cytometry on day 1 c * p < 0.05, ** p < 0.01; *** p < 0.001; NS, non-significance compared with Control cells