Fig. 3

BMP2K promotes proliferation in response to MLN8237 challenge. Control or BMP2K overexpression cells were cultured in basal conditions (a) or in the presence of MLN8237 (0.5 μM) for 6 h followed by wash and reseeding in fresh medium (b) for a consecutive 3 days. The cell numbers were counted on each day for cell proliferation assay. c The MLN8237-treated cells were further labeled with BrdU and harvested for cell cycle profile analysis by staining BrdU with a fluorescence-conjugated BrdU antibody and staining DNA with DAPI. The right panel is the statistics of the cell cycle profile. d–f The positive effect of BMP2K knockdown (shBMP2K#2) on cell proliferation were detected and cell cycle profile was analyzed as described in A-C. * p < 0.05, ** p < 0.01; *** p < 0.001; NS, non-significance compared with Control or Scramble cells