Fig. 2

BMP2K inhibits MLN8237-induced megakaryocytic differentiation. a BMP2K protein level in Control and BMP2K overexpression cells was confirmed by immunoblotting (left panel). The right panel is the statistics of the densitometric analysis of the immunoblotting. bBMP2K mRNA level in these cells was measured by quantitative RT-PCR. c–d Control and BMP2K overexpression cells were treated with Vehicle (dot line) or MLN8237 (solid line) for 48 h. The megakaryocytic differentiation was measured by CD41 staining (c) or by DAPI staining for DNA content (d). e–h BMP2K downregulation by shRNAs (shBMP2K#1 and shBMP2K#2) in CMK cells and its positive effect on MLN8237-induced megakaryocytic differentiation was measured as described in a–d. Gates in D and H represent cells with ploidy ≥ 8 N. * p < 0.05, ** p < 0.01; *** p < 0.001; NS, non-significance compared with Control or Scramble cells