Fig. 1

Bioinformatic analysis of the mRNA expression alteration based on HBE cells with or without CRISPR/Cas9-mediated p53-knockout. a p53 knockout efficiency was determined using western blotting. b Quantitative measurement of differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated p53-knockout (p53-wt) after 4 Gy IR. c. A heatmap was illustrated to show the differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated p53-knockout (p53-wt) after 4 Gy IR. Pink represents upregulated mRNA, and purple represents down-regulated mRNA. d A volcano map was illustrated to show the differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated p53-knockout (p53-wt) after 4 Gy IR. Red represents upregulated mRNA, green represents down-regulated mRNA and grey represents the mRNA without expression change