Fig. 1

OTUB1 was elevated in RCC tissues and cell lines. a The expression levels of OTUB1 in RCC tissues and adjacent noncancer tissues were detected by qRT-PCR (N = 67). ** represents Tumor vs. Normal tissues, P < 0.01. b Immunohistochemistry analysis of adjacent noncancer tissues and low grade (I + II) or high grade (III + IV) RCC tissues. c The expression levels of OTUB1 in RCC cell lines (Caki-1, ACHN, A-498 and 786-O), HK2 and HUVEC were detected by qRT-PCR (N = 67). ** represents RCC cell lines vs. HK2, P < 0.01. d The expression levels of OTUB1 in RCC cell lines (Caki-1, ACHN, A-498 and 786-O), HK2 and HUVEC were detected by western blot. ** represents RCC cell lines vs. HK2, P < 0.01