Fig. 3
From: HCC-derived EGFR mutants are functioning, EGF-dependent, and erlotinib-resistant
Protein Expression of downstream signaling molecules (AKT, phosphorylated AKT, ERK, and phosphorylated ERK). NIH-3T3 cells harboring pBabe-puro, EGFR WT and mutants were starved with serum-free media for 24 h and were then treated with or without 50 ng/ml EGF for 30 min. Protein extracts of these cells were analyzed by immunoblotting for downstream signaling molecules, as described in the “Materials and methods” section, β-actin used as a loading control (a). Graphic presentations of relative band intensities of these downstream signaling molecules of each sample are shown as mean ± SEM (b, c)