Fig. 2

Protein Expression of EGFR and phosphorylated EGFR at Tyr869, Tyr1092. NIH-3T3 cells harboring pBabe-puro, EGFR WT and mutants were starved with serum-free media for 24 h and were then treated with or without 50 ng/ml EGF for 30 min. Protein extracts of these cells were analyzed by immunoblotting for EGFR and phosphorylated EGFR at Tyr869 and Tyr1092 [pEGFR (Y869), pEGFR (Y1092)], as described in the “Materials and methods” section, β-actin used as a loading control (a). Graphic presentations of relative band intensities of Tyr869 and Tyr1092 of each sample are shown as mean ± SEM (b, c respectively)