Fig. 5

The synthesis of ceramides and complex sphingolipids is not required for exocytosis. a Localization of the exocyst components in cells treated with either Aureobasidin A or Fumonisin B1. Wild-type cells harboring SEC3-GFP, SEC5-GFP, SEC8-GFP or EXO70-GFP on the chromosome were grown to early log phase in YPD liquid medium overnight at 25 °C. The cells were then treated with 10 μg/ml Aureobasidin A or 500 μM Fumonisin B1 for 120 min. 2 µl of the suspension was dropped onto the microslide for microscopy. Scale bar, 2 μm. b Statistical analysis was performed for the percentage of polarized exocyst localization in each yeast strains a. n = 3. *p < 0.01. c Bgl2 secretion in wild type cells treated with or without Aureobasidin A. Wild-type cells were grown to early log phase in YPD liquid medium overnight at 25 °C. Aureobasidin A was added into the medium to a final concentration of 10 μg/ml, and the cells were incubated at 25 °C for 120 min. External and internal Bgl2 secretion was analyzed by western blot using anti-Bgl2 antibody. The total protein on PVDF membranes were stained with Ponceau S as loading control. d Bgl2 secretion in wild type cells treated with or without Fumonisin B1. Wild-type cells were grown to early log phase in YPD liquid medium overnight at 25 °C. Fumonisin B1 was added into the medium to a final concentration of 500 μM, and the cells were incubated at 25 °C for 120 min