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Fig. 4 | Cell & Bioscience

Fig. 4

From: Sphingolipids are required for exocyst polarity and exocytic secretion in Saccharomyces cerevisiae

Fig. 4

Secretory vesicles were accumulated in lcb1-100 mutant cells at restrictive temperature. a Wild-type and lcb1-100 mutant cells were grown to early log phase in YPD liquid medium overnight at 25 °C and then shift to 39 °C for 60 min. The cells were fixed, stained, and examined by thin-section electron microscopy. b Statistical analysis of vesicle accumulation in wild type and lcb1-100 mutant cells in a. n = 15. *p < 0.01. “Ve”, vesicle. c Diameter measurement of vesicles accumulated in lcb1-100. n = 600. d Histogram of vesicle diameter distribution in the lcb1-100 mutant cell shown in c

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