Fig. 3

Localization of Sec4 in wild-type and lcb1-100 mutant cells. a Wild type and lcb1-100 mutant cells were grown to early log phase at 25 °C. The cells were then shifted from 25 to 39 °C for 60 min before immuno-fluorescence microscopy. Sec4 is polarized in wild type cells, while it is dispersed in lcb1-100 mutant cells at restrictive temperature. b Statistical analysis was performed on the percentage of polarized localization of Sec4 in yeast strains in a. n = 3. Three independent experiments were performed. *p < 0.01