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Fig. 1 | Cell & Bioscience

Fig. 1

From: Sphingolipids are required for exocyst polarity and exocytic secretion in Saccharomyces cerevisiae

Fig. 1

Localization of the exocyst components in lcb1-100 mutant cells and in cells treated with myriocin. a The yeast strains harboring SEC3-GFP, SEC5-GFP, SEC8-GFP or EXO70-GFP on the chromosome were grown to early log phase in YPD liquid medium overnight at 25 °C. The cells were then shifted from 25 to 39 °C for 60 min before fluorescence microscopy. GFP-tagged Sec3, Sec5, Sec8 and Exo70 remained polarized in buds of wild-type cells at both 25 °C and 39 °C. The exocyst components in lcb1-100 mutant cells were located to bud at 25 °C but dispersed in cytoplasm at 39 °C after the temperature shift. Scale bar, 2 μm. b Statistical analysis was performed on the percentage of polarized localization in each yeast strains at 25 °C and 39 °C in a. n = 3. Three independent experiments were performed. *p < 0.01. c Wild-type cells harboring SEC3-GFP, SEC5-GFP, SEC8-GFP or EXO70-GFP on the chromosome were grown to early log phase in YPD liquid medium overnight at 25 °C. The cells were then treated with or without 10 μg/ml myriocin for 60 min. 2 µl of the suspension was dropped onto the microslide for microscopy. Scale bar, 2 μm. d Statistical analysis was performed for the percentage of polarized exocyst localization in each yeast strains a. n = 3. Three independent experiments were performed. *p < 0.01

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