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Fig. 3 | Cell & Bioscience

Fig. 3

From: Serum IP-10 and IL-17 from Kawasaki disease patients induce calcification-related genes and proteins in human coronary artery smooth muscle cells in vitro

Fig. 3

IP-10/IL-17 co-treatment induces OPN, OCN and ALP expressions in HCASMCs. The HCASMCs were maintained either as controls or were treated with IP-10, IL-17, or both, for 3, 7, or 10 days, after which we examined the mRNA expressions of OPN (a), OCN (b), and ALP (c) using real-time PCR and the protein expressions of OPN, OCN, and ALP (de) using the western blot test. The data in (ae) are mean ± SEM from three independent experiments. * P < 0.05 vs. control cells. The results in (de) are representative of three independent experiments with similar results

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