Fig. 1
From: The replisome guides nucleosome assembly during DNA replication
The replisome provides a guide to modulate DNA replication-coupled nucleosome disassembly and assembly. When the replication fork is moving forward, the replicative helicase Mcm2 might cooperate with the histone chaperone Asf1 or FACT to facilitate the disassembly of parental nucleosomes and the recycling of parental histones. Dpb3-Dpb4 (POLE3-POLE4), the subunits of DNA Pol, provide a guide for the assembly of parental histones on the leading strand. The Mcm2-Ctf4-Pol α axis directs the recycling of the parental histone to the lagging strand. RPA-ssDNA functions as a general platform that directs the histone chaperones (CAF-1, Rtt106 and FACT) carrying newly synthesized histones to enter the replication fork, thereby modulating nucleosome assembly on daughter strands. PCNA marks nascent DNA and promotes CAF-1-mediated nucleosome assembly. In intriguing findings, the main PCNA loader Rfc1-RFC interacts with Asf1, and the PCNA unloader Elg1-RFC interacts with Rtt106