Fig. 3

RT-qPCR validation of hsa_circRNA_102488. a Schematic representation of the UBA52 gene, origin of hsa_circRNA_102488. The graph shows the map of hsa_circRNA_102488 which is originated from exons 2 and 3 of its host mRNA (NCBI RefSeq track shown in the UCSC Genome Browser). b hsa_circRNA_102488 backsplicing. The Sanger-Sequencing electropherogram specifies the sequence of the backspliced junction that results in the circRNA formation. c–e hsa_circRNA_102488 and UBA52 mRNA expression levels between atherotrombotic vs cardioembolic samples. Percentage of hsa_circRNA_102488 expression relative to GAPDH mRNA (c) or UBA52 mRNA (d) is decreased in atherotrombotic with respect to cardioembolic stroke patients. However, we found similar expression levels of UBA52 mRNA in atherotrombotic and cardioembolic samples groups (e). Error bars: 95% CI. **p-value < 0.01; ***p-value < 0.001