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Fig. 5 | Cell & Bioscience

Fig. 5

From: Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway

Fig. 5

Dynorphin activation of KOR promoted microglial M2 polarization via inhibiting TLR4/NF-κB pathway in LPS-stimulated BV-2 microglial cells. a Determination of TLR4, IκB-α, p-IκB-α, NF-κB p65, and p-NF-κB p65 protein levels by western blot in BV-2 cells in the groups of Control, LPS, LPS + Dyn-A, LPS + GNT1, and LPS + Dyn-A + GNT1. b Determination of CD16/32 and CD206 expression by flow cytometry and c levels of M1-related cytokines (IL-1β and IL-6) and M2-related cytokines (IL-4 and IL-10) by ELISA in LPS-stimulated BV-2 cells in the groups of LV-NC, LV-TLR4, LV-NF-κB, Dyn-A + LV-NC, Dyn-A + LV-TLR4, and Dyn-A + LV-NF-κB. N = 3. Dyn-A, Dynorphin-A. *p < 0.05, **p < 0.01, vs. Control or NC group, #p < 0.05, ##p < 0.01, vs. LPS or LV-TLR4 group; &p < 0.05, &&p < 0.01, vs. LPS + Dyn-A or LV-NF-κB group

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