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Fig. 4 | Cell & Bioscience

Fig. 4

From: Critical roles of microRNA-141-3p and CHD8 in hypoxia/reoxygenation-induced cardiomyocyte apoptosis

Fig. 4

Knockdown of CHD8 inhibits apoptosis induced by H/R in H9c2 cardiomyocytes. H9c2 cardiomyocytes were transfected with CHD8-siRNA (CHD8-Si) or negative control-siRNA (NC-Si) for 6 h in low glucose DMEM, then cultured for another 48 h in basic DMEM. Transfected H9c2 cardiomyocytes were subjected to hypoxia treatment for 8 h, then reoxygenated for 48 h as in Fig. 1. a The expression of Cl-caspase-3 and caspase-3 was determined by Western blot. b Apoptotic cells were determined by flow cytometric analysis. N = 3 per group. *P < 0.05, **P < 0.01, compared with indicated groups

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