Fig. 2

Analysis of differentially expressed miRNAs in HCC patients with HBV-negative infection and HBV-positive infection using high-throughput sequencing. a The total, upregulated, and downregulated differentially expressed miRNAs. b GO analysis of the differentially expressed miRNAs. The y-axis represents the number of genes and the x-axis represents the GO terms. c Hierarchical clustering (heatmap) indicating the differences in miRNA expression profiling between the two groups. Green and red represent the downregulated and upregulated miRNAs, respectively. d Volcano plot showing the expression profiling in the two groups. The vertical green lines refer to a 2.0-fold (log2 scaled) upregulation and downregulation, respectively. The horizontal green line corresponds to a p-value of 0.05 (− log10 scaled). The red points in the plot represent differentially expressed miRNAs with statistical significance. e KEGG analysis of the differentially expressed miRNAs