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Fig. 4 | Cell & Bioscience

Fig. 4

From: The antagonism of 6-shogaol in high-glucose-activated NLRP3 inflammasome and consequent calcification of human artery smooth muscle cells

Fig. 4

Akt signaling in HASMCs regulates HG-triggered ROS production, NLRP3 inflammasome and subsequent calcification. a HASMCs were kept as the controls or were treated with NG, mannitol (M), or HG for 4 h and then the Akt phosphorylation was examined. bd HASMCs were kept as the controls or were pretreated with (i) DMSO or LY294008, an akt phosphorylation inhibitor, or (ii) pcDNA empty vector (EV) or dominant negative (dn)-Akt-expressed plasmid and then treated with HG for 2 and 14 days. The NLRP3 and IL-1β (pro and mature form) protein expressions b, ROS production c, and OPN, OCN, and ALP mRNA expressions c in HASMCs, and HASMC calcification d were examined. Results in a, b are representative of three independent experiments with similar results and the bar graphs are mean ± SEM from three independent experiments. Data in c, d are mean ± SEM from three independent experiments. *P < 0.05 vs. control cells. #P < 0.05 vs. DMSO/ or EV/HG-treated cells

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