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Fig. 1 | Cell & Bioscience

Fig. 1

From: The antagonism of 6-shogaol in high-glucose-activated NLRP3 inflammasome and consequent calcification of human artery smooth muscle cells

Fig. 1

HG initiates HASMC calcification. ad HASMCs were kept as the controls or were treated with 5 mM (normal concentration of glucose, NG) or 25 mM (high concentration of glucose, HG) glucose or 25 mM mannitol (M) for 3, 7, and 14 days and then the mRNA ac and protein d expressions of OPN, OCN and ALP were examined. e HASMCs were kept as the controls or were treated with NG, mannitol, or HG for 14 days and then the HASMC calcification was examined by ARS stain and were detected at 405 nm. Data in ac and e are mean ± SEM from three independent experiments. Results in d are representative of three independent experiments with similar results and the bar graphs are mean ± SEM from three independent experiments. *P < 0.05 vs. control cells

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