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Fig. 4 | Cell & Bioscience

Fig. 4

From: SNHG6 modulates oxidized low-density lipoprotein-induced endothelial cells injury through miR-135a-5p/ROCK in atherosclerosis

Fig. 4

SNHG6 regulated ox-LDL-induced injury of HUVECs via interacting with miR-135a-5p. ox-LDL-induced HUVECs were transfected with si-NC, si-SNHG6#1, si-SNHG6#1 + miR-NC inhibitors, si-SNHG6#1 + miR-135a-5p inhibitors for 48 h. a The enrichment of miR-135a-5p was examined by RT-qPCR. b Cell viability was determined by CCK-8 assay. c Caspase-3 activity was assessed by using commercially-available kit. d Cell apoptosis was detected by flow cytometry. e The protein expression of Bax and Bcl-2 was examined by western blot assay. f, g The release of IL-6 and TNF-α were measured by ELISA assay. h, i The production of ROS and MDA were detected using commercially-available kits. **P < 0.01; #P < 0.05 and ##P < 0.01

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