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Fig. 2 | Cell & Bioscience

Fig. 2

From: SNHG6 modulates oxidized low-density lipoprotein-induced endothelial cells injury through miR-135a-5p/ROCK in atherosclerosis

Fig. 2

Knockdown of SNHG6 alleviated ox-LDL-induced injury of HUVECs. a The abundance of SNHG6 in HUVECs transfected with si-NC, si-SNHG6#1 or si-SNHG6#2 was examined by RT-qPCR. HUVECs were transfected with si-NC, si-SNHG6#1, or si-SNHG6#2 and then treated with or without 25 µg/ml ox-LDL for 24 h. b The expression of SNHG6 was detected by RT-qPCR assay. c Cell viability was determined by CCK-8 assay. d Caspase-3 activity was measured using commercially-available kit. e Cell apoptosis was assessed by flow cytometry. f The expression of apoptosis-related protein Bax and Bcl-2 was examined by western blot assay. g, h The release of inflammatory cytokines IL-6 and TNF-α were assessed by ELISA assay. i, j The production of oxidative status markers ROS and MDA were determined using commercially-available kits. *P < 0.05 and **P < 0.01; #P < 0.05 and ##P < 0.01

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