From: Epigenetic roles of PIWI proteins and piRNAs in lung cancer
PIWI | Up/Down | Tissue/cell line | Technique | Final result | Ref |
---|---|---|---|---|---|
PIWIL1 | Up | H23 and A549 cell lines | Real-time PCR/immunohistochemistry/ELISA assay | Decrease in survival time of patients and shorten time to relapse | [27] |
PIWIL4 | Down | H23 and A549 cell lines | Real-time PCR/immunohistochemistry/ELISA assay | Increase of transposon activities and genomic instability/decrease in survival time of patients and shorten time to relapse | [27] |
PIWIL2 | Up | A549 and H460 cell lines/nude mice | Real-time PCR/western blot/immunofluorescence staining/immunohistochemistry/flow cytometry | Proliferation induction/tumor growth promoting/apoptosis inhibition | [36] |
HIWI | Up | SSClo Aldebr cells/SPC-A1 cell line/mice | Hiwi shRNA plasmids treatment/immunohistochemistry/enzyme immunoassay/flow cytometry | Increasing proliferation/apoptosis reduction | |
HIWI | Up | A549 cell lines | Real-time PCR/western blot/gain of function and loss of function strategies/colony formation assay/cell counting assay | Cell proliferation increasing | [38] |
PIWIL1 | Up | A549 and H1299 cell lines | Real-time PCR/gain of function and loss of function strategies/western blot/bioinformatics analysis | Enhancing proliferation, migration and invasion | [39] |
PIWIL1 | Up | A549/NCIH1299/CRL- 9482 | Microarray analysis/real-time PCR/western blot | Increase in cell growth and proliferation | [40] |