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Fig. 8 | Cell & Bioscience

Fig. 8

From: Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier

Fig. 8

The capacity of human amniotic fluid stem cells (hAFSCs) to differentiate into osteogenic, adipogenic, and chondrogenic lineages. a In culture, hAFSCs have fibroblast-like morphology. b Adipogenic differentiation was evaluated using Oil Red O staining. Cytoplasmic lipid droplets appear red (×400). c, d Osteogenic differentiation was confirmed by Alizarin red and alkaline phosphatase staining. Calcium deposits and alkaline phosphatase activity appear red (×100 and ×400, respectively). e, f Chondrogenic differentiation was confirmed by hematoxylin and eosin and immunofluorescence staining using specific monoclonal antibodies against type II collagen (×200 and ×400, respectively) [30]. Collagenase I isolated cells differentiation figures: g Adipogenic differentiation was evaluated using Oil Red O staining. h Osteogenic differentiation was confirmed by Alizarin red and alkaline phosphatase staining. i Chondrogenic differentiation was confirmed by staining of Alcian blue [35]

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