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Fig. 4 | Cell & Bioscience

Fig. 4

From: Drosophila Prominin-like, a homolog of CD133, interacts with ND20 to maintain mitochondrial function

Fig. 4

Knock down of prominin-like leads to mitochondrial dysfunction. a Transmission electron microscopy (TEM) images of S2 cells of Prominin-like knock down and control cells. Scale bars: 0.2 μm. a′ Statistical analysis of the rate of swelling mitochondria in prominin-like knock down cells (day2, n = 25; day 4, n = 26) and control cells (n = 21). b, b′ S2 cells were incubated with prominin-like dsRNA for 4 days. The protein expression levels of ATP5a, COX IV, NDUFS3, ND20 and VDAC were determined each day after RNAi treatment by Western blotting. β-tubulin expression was determined as a loading control under the same conditions. c Intensity measurement of the Western blot result in b by Image J software. d TEM images of prominin-like mutants and the control flies at various time points. The mitochondrial morphologies of 5-day-old and 20-day-old prominin-like mutants were similar to those of the control animals; while in 45-day-old mutants, the mitochondria showed obviously disordered mitochondrial cristae (arrows). e The protein expression levels of VDAC of prominin-like mutants and the control flies at vinous time points were determined by western blotting. e′ Intensity measurement of the western blot result in e by Image J software; Data showed that VDAC levels of prominin-like mutants were down-regulated in 45-day-old mutants not 5-day-old mutants. b The protein expression levels of ND20 of prominin-like mutants and the control flies at various time points were determined by western blotting. f′ Intensity measurement of the western blot result in f using Image J software. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01

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