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Fig. 3 | Cell & Bioscience

Fig. 3

From: Drosophila Prominin-like, a homolog of CD133, interacts with ND20 to maintain mitochondrial function

Fig. 3

The deficiency of Prominin-like influences cell growth and ATP production. a Quantitative real-time PCR was used to analyze prominin-like mRNA levels and to test the efficiency of the dsRNA treatment for 4 days in S2 cells. b Western blot examination of Prominin-like protein levels after prominin-like dsRNAi treatment for 4 days. c Cell growth curve analysis showed that upon the knocking down of prominin-like, cell growth was delayed compared with the control cells. d Cellular ATP levels were measured using a bioluminescence assay, showing significant reductions of ATP levels in prominin-like RNAi-treated cells at day 2 and day 3 compared to control cells. The levels then recovered to almost normal at the third day after RNAi treatment (P value in the and third day was 0.759 and in the fourth day was 0.2492). d′ Measurement of mitochondrial ATP levels. Data showed significant reductions of mitochondrial ATP levels in prominin-like RNAi-treated cells at day 1–3 compared to control cells. The levels then gradually recovered to normal levels at the fourth day after RNAi treatment. e The rates of cellular ROS generation from prominin-like deficiency S2 cells and control cells. The result showed that the ROS production was upregulated in the short-term. e′ Measurement of mitochondrial ROS. Ratio of geometric mean intensity between levels of the ROS generation in the prominin-like knock down cells and control cells. Data showed significant increase of mitochondrial ROS levels in prominin-like RNAi-treated cells at day 1–2 compared to control cells. The levels then gradually recovered to normal levels at the fourth day after RNAi treatment. f Analysis of mitochondrial membrane potential (Δψm). Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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