Fig. 1

Structural characteristics and the cellular localization of Prominin-like. a Multiple sequence alignments of CD133 from five species; Daniorerio, Xenopus tropicalis, Homo sapiens, Musmusculus and Prominin-like from Drosophila melanogaster. Identical residues are marked with black stars, conserved residues with a blue background, and similar residues with a salmon background. Predicted transmembrane domains are underlined. Potential N-glycosylation sites are highlighted in triangles. b Membrane topology of Drosophila Prominin-like. Seven potential N-glycosylation sites have been drawn. c Western blot analysis of Prominin-like levels in untreated S2 cells. d S2 cells were transiently transfected with Flag-tagged Prominin-like, and colabeled with anti-Flag antibody (green), MitoTracker Red (the upper lane)/VDAC (the middle lane)/Cytochrome C (the lower lane) (red) and DAPI (blue). The merge of the green and red signals (yellow) confirms the partially mitochondrial localization of Prominin-like. e–g Western blot analysis of cytosolic and mitochondrial fractions separated by centrifugation. e Samples were probed with anti-Prominin-like, anti-ATP5a, and anti-tubulin antibodies. f Samples were probed with anti-Prominin-like, anti-Cytochrome C, and anti-tubulin antibodies. g Samples were probed with anti-Prominin-like, anti-LAMP1, and anti-tubulin antibodies