Fig. 2
From: Immunosuppressive effects of a novel potassium channel toxin Ktx-Sp2 from Scorpiops Pocoki
The expression, purification and identification of peptide Ktx-Sp2. a Purification of Ktx-Sp2 by HPLC on a C18 column. b Mass spectrum of Ktx-Sp2 peptide measured by MALDI-TOF–MS. Measured value is 3928.0 Da, and the calculated one is 3928.0 Da. Right inset shows the tricine/SDS-PAGE analysis of the purification of Ktx-Sp2 peptide. M, molecular mass markers; Lane 1, proteins from non-induced E. coli Rosetta (DE3) cells; lane 2, proteins from induced E. coli Rosetta (DE3) cell containing pGEX-4T-1-Ktx-Sp2 by IPTG; lane 3, spurified GST fusion protein after affinity chromatography and desalting; lane 4, fusion protein cleaved by enterokinase; lane 5, purified Ktx-Sp2 by reversed phase HPLC