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Fig. 3 | Cell & Bioscience

Fig. 3

From: PRL2 serves as a negative regulator in cell adaptation to oxidative stress

Fig. 3

PRL2 negatively regulates cell survival under oxidative stress via PDK1/AKT signaling. a Bone marrow cells from PRL2+/+ and PRL2−/− mice were treated with 50 μM H2O2 for different time. Cell lysates were subjected to SDS-PAGE followed by immunoblot analysis with indicated antibodies. b 293T stable cell line with or without PRL2 overexpression were treated with 50 μM H2O2 for different time. Cell lysates were subjected to SDS-PAGE followed by immunoblot analysis with indicated antibodies. c Prior to oxidative stress exposure, 293T cells were pre-treated with 10 μM SC79 (AKT activator) for 30 min. Relative cell viability was measured by the CCK-8 assay following oxidative stress induced by various concentrations of H2O2. The experiments in this figure were repeated at least three times with similar results. Error bars represent the SEM. Statistics were performed on pooled data from 2 independent experiments. **p < 0.01, ***p < 0.005

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