Fig. 2

PRL2 senses oxidative stress via highly reactive cysteine residues. a PRL2 proteins were treated with DTT or H₂O₂ then were subjected to SDS-PAGE under reductive and oxidative conditions. b 293T cells were treated with various concentrations of H₂O₂ for 30 min. Some were pre-treated with cycloheximide for 1 h prior to oxidative stress exposure. Cell lysates were subjected to SDS-PAGE under reductive and oxidative conditions followed by immunoblot analysis with indicated antibodies. c 293T cells were transfected with pRK5. 24 h after transfection, cells were treated with various concentrations of H₂O₂ for 30 min. Cell lysates were subjected to SDS-PAGE under reducing and oxidative conditions followed by immunoblot analysis with indicated antibodies. The experiments in this figure were repeated at least three times with similar results. *p < 0.05, **p < 0.01, ***p < 0.005