Fig. 3From: CRISPRa-mediated FOXP3 gene upregulation in mammalian cellsUpregulation of FOXP3 mRNA in Jurkat cells via targeting of selected promoter and enhancer regions. Jurkat cells were electroporated with plasmids encoding indicated single sgRNAs or a combination of sgRNAs in combination with the dCas9-VPR transcriptional activator and fold activation of FOXP3 gene expression was measured in reference to a control sample, electroporated with plasmids encoding random sgRNA (rnd) and dCas9-VPR. Each experiment was repeated 3 times. Cells were lysed and RNA isolated 48 h post electroporation. a Jurkat cells were electroporated with a combination of four sgRNAs targeting each region [Core (Fox 1–4), CNS1 (Fox 5–8), CNS2 (Fox 9–12), CNS3 (Fox 13–16), Cage1 (Fox 17–20) and Cage2 (Fox 21–24)] and FOXP3 gene expression was measured. b Jurkat cells were electroporated with each sgRNA (Fox 1–24) separately and the effect of each single sgRNA on FOXP3 gene expression was measured. c Jurkat cells were electroporated with sgRNAs 1, 14, 15, 17 and 18 separately and in combination and FOXP3 gene expression was measuredBack to article page