Fig. 6

The pipeline of this study. The RNAseq data and clinical data for lung adenocarcinoma were first downloaded from the TCGA. RNA-seq data were used to analyze gene differential expression, and perform GO and KEGG functional analysis. Clinical data combined with lymph node metastasis and TNM analysis of differential genes. And the gene set associated with both lymph node metastasis and TNM stage was obtained. Then the survivals of the intersection genes were analyzed by Kruskal–Wallis algorithm to find the target gene. The function of the single gene GSEA of the target gene was then studied. The prognosis between the gene and the clinical variable was verified by the Cox regression analysis of single gene and multivariables. Finally, in the CCLE database, the co-expression genes of the target gene and the enrichment and signaling pathway analysis in which these genes are involved are further verified