Fig. 3
From: Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma
Circ_0051079 negatively regulated miR-26a-5p in osteosarcoma cells. a The putative sequences of miR-26-5p and circ_0051079 binding site. b, c Luciferase activity of circ_0051079 in osteosarcoma cells. Cells were transfected with miR-26a-5p and the plasmids with the circ_0051079 reporter and then luciferase activity was analyzed by dual-luciferase system. d MiR-26a-5p expression in osteosarcoma cells. The cells were transfected with circ_0051079 shRNA for 48 h and the levels of it was detected by real time RT-PCR. e Circ_0051079 expression in osteosarcoma cells. The cells were transfected with miR-26a-5p mimics for 48 h and the levels of it was detected by real time RT-PCR. f MiR-26a-5p expression in osteosarcoma tissues. Total RNA from 32 primary osteosarcoma tissues was extracted for miR-26a-5p examination by real time RT-PCR. **p < 0.01