Fig. 5From: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editinga The disruption rate induced by injection of Cas9/sgRNAs into embryos of X. tropicalis by T7E1 method assay. “−” represents without T7 enzyme digestion; “+” represents plus T7 enzymedigestion; and “M” represents DNA marker. b–d The disruption rate induced by injection of Cas9/sgRNAs into embryos of X. tropicalis using TA cloning and sequencing analysis. After TA clonging, 20 TA clones were randomly picked up for DNA sequencing, and the disruption rate (%) was calculated based on the results of DNA sequencingBack to article page