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Fig. 5 | Cell & Bioscience

Fig. 5

From: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

Fig. 5

a The disruption rate induced by injection of Cas9/sgRNAs into embryos of X. tropicalis by T7E1 method assay. “−” represents without T7 enzyme digestion; “+” represents plus T7 enzymedigestion; and “M” represents DNA marker. bd The disruption rate induced by injection of Cas9/sgRNAs into embryos of X. tropicalis using TA cloning and sequencing analysis. After TA clonging, 20 TA clones were randomly picked up for DNA sequencing, and the disruption rate (%) was calculated based on the results of DNA sequencing

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