Fig. 5

LncRNA H19 over-expression suppressed Th17 differentiation and ESC proliferation through miR-342-3p/IER3. a miR-342-3p inhibitor suppressed IL-17 level, the percentage of Th17 cells/CD4+ T cells, and ESC viability. b CD4+ T cells were divided into lenti-NC, lenti-H19, lenti-H19 + pre-NC, lenti-H19 + miR-342-3p mimic groups. Then, CD4+ T cells were co-cultured with ESC for 48 h. The viability of ESC was detected by MTT assay and resazurin assay. c IL-17 level was detected using ELISA. The percentage of Th17 cells/CD4+ T cells was detected using flow cytometry. TH17 cell marker RORγt expression was detected by western blot. *p < 0.05, compared with NC or lenti-NC; ^#p < 0.05, compared with lenti-H19 + pre-NC