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Fig. 4 | Cell & Bioscience

Fig. 4

From: LncRNA H19 over-expression inhibited Th17 cell differentiation to relieve endometriosis through miR-342-3p/IER3 pathway

Fig. 4

LncRNA H19 over-expression regulated miR-342-3p/IER3 expression. a Bioinformatics software predicted the miR-342-3p targeted to 3′UTR of IER3. miR-342-3p mimic or inhibitor were transfected into HEK293 cells to detect the luciferase activity. IER3 mRNA and protein levels were detected by qRT-PCR or Western blot after miR-342-3p mimic or inhibitor treatment. b CD4+ T cells were divided into lenti-NC, lenti-H19, lenti-H19 + pre-NC, lenti-H19 + miR-342-3p mimic groups. miR-342-3p expression and IER3 protein level were detected by qRT-PCR and Western blot. *p < 0.05, compared with pre-NC or lenti-NC; #p < 0.05, compared with lenti-H19 + pre-NC

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