Fig. 5

lnc-DILC inhibits the interaction between PTEN and WWP2. a ACHN cells transfected with pcDNC3.1-lnc-DILC or the empty vector were treated with MG132 for 4 h before harvest. PTEN was immunoprecipitated and immunoblotted with the indicated antibodies. b Caki-1 cells transfected with lnc-DILC or negative control siRNAs were treated with MG132 for 4 h before harvest. PTEN was immunoprecipitated and immunoblotted with the indicated antibodies. c ACHN cells cotransfected with pcDNC3.1-lnc-DILC and pcDNA3.1-WWP2 were treated with MG132 for 4 h before harvest. PTEN was immunoprecipitated and immunoblotted with the indicated antibodies. d ACHN and Caki-1 cells with lnc-DILC alteration were cotransfected with pcDNA3.1-WWP2 and WWP2 siRNAs, respectively. 48 h later, the PTEN was detected by western blot assay. e The effect of lnc-DILC overexpression on the interaction between PTEN and WWP2 was determined by co-immunoprecipitation assay in ACHN cells. f The effect of lnc-DILC knockdown on the interaction between PTEN and WWP2 was determined by co-immunoprecipitation assay in Caki-1 cells