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Fig. 7 | Cell & Bioscience

Fig. 7

From: KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes

Fig. 7

Inhibition of endocytosis and endosomal trafficking does not have a significant effect in cytokinesis in ATDC5 cells. a Quantification of bi- and multi-nucleation rate in GFP and GFP-dynamin 2 K44A positive cells (n = 5 independent experiments). P = 0.4373; unpaired two-tailed t-test. Data are mean ± S.D. b Absence of transferrin (red) uptake in GFP-dynamin 2 K44A positive cells (green) showing inhibition of clathrin-dependent endocytosis. Scale bar: 20 μm. c Quantification of bi- and multi-nucleation in GFP and GFP-Rab11 S25N positive cells (n = 5 independent experiments). P = 0.8207; unpaired two-tailed t-test. Data are mean ± S.D. d Transferrin (red) uptake in sh-ctl and sh-Kif5b ATDC5 cells. Scale bar: 20 μm

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