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Fig. 5 | Cell & Bioscience

Fig. 5

From: KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes

Fig. 5

KIF5B localizes to central spindle during cytokinesis in chondrocytes. a Immunofluorescence of KIF5B (green) and α-tubulin (red) in ATDC5 cells in different phases of mitosis. Scale bar: 10 μm. b Immunofluorescence of KIF5B (green) and α-tubulin (red) in sh-Kif5b knockdown ATDC cells (clone #4) in cytokinesis. Scale bar: 10 μm. c Immunofluorescence of KIF5B (green) and α-tubulin (red) in primary chondrocytes in late cytokinesis. Scale bar: 10 μm. d Midbody isolation using ATDC5 cell lines. Immunofluorescence of α-tubulin showing the isolated midbody (left panel). Scale bar: 5 μm. Western Blot showing that KIF5B, α-tubulin and Aurora B kinase were present in midbody fraction (right panel)

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