Fig. 2

KIF5B deficient chondrocytes display cytokinetic defect. a H & E staining on sections of the proximal tibial growth plate of P1 newborns showing binucleated cells in mutant growth plates. Scale bar: 5 μm. b Cytospin of isolated chondrocytes from the proliferating zone of tibia and femur of P1 newborns were stained with phalloidin (green) and DAPI (blue). Asterisks denote typical binucleated cells. Scale bar: 20 μm. c Quantification of bi- and multi-nucleated rate in cytospin preparation samples from P1 newborns (Kif5b^fl/+: n = 5; Col2cre; Kif5b^fl/−: n = 5). ***P < 0.0001; unpaired two-tailed t-test. Data are mean ± S.D. d Time-lapse images of primary chondrocytes in mitosis. Scale bar: 10 μm. e Quantification of cytokinesis duration of primary chondrocytes (Kif5b^fl/+ cells: n = 232; Col2cre; Kif5b^fl/− cells: n = 231). ***P < 0.0001; two-tailed Mann–Whitney U-test. The whisker plot shows median (lines), interquartile range (boxes) and 5% to 95% percentile (whiskers). Duration of cytokinesis was calculated from the furrow ingression to the final separation of the two daughter cells. Cells fusing back were not included in this analysis