Fig. 1

Col2a1-Cre directed depletion of KIF5B leads to growth plate abnormalities. a Representative X-ray images of the Kif5b^fl/+ and Col2cre; Kif5b^fl/− mice at 6 weeks of age. b Length of long bones of Kif5b^fl/+ and Col2cre; Kif5b^fl/− mice at 6 weeks of age (Kif5b^fl/+: n = 5; Col2cre; Kif5b^fl/−: n = 5). ***P < 0.0001; unpaired two-tailed t-test. Data are shown as mean ± S.D. c, d H & E staining on sections of the proximal tibial growth plate of P1 newborns. e, f Enlarged images showing absence of columnar structure the mutant growth plate. g–j In situ hybridization of Col2a1 and Col10a1 probes on adjacent sections for H & E staining. Scale bar (c–j): 200 μm. k Quantification of BrdU-positive chondrocytes in the entire proximal tibial growth plate of P1 newborns (Kif5b^fl/+: n = 3; Col2cre; Kif5b^fl/−: n = 3). Three sections were counted for each sample. **P = 0.0092; unpaired two-tailed t-test. Data are mean ± S.D. l Quantification of TUNEL-positive chondrocytes in the entire proximal tibial growth plate of P1 newborns (Kif5b^fl/+: n = 3; Col2cre; Kif5b^fl/−: n = 3). Three sections were counted for each sample. ***P = 0.0003; unpaired two-tailed t-test. m TUNEL assay on sections of the proximal tibial growth plate of P1 newborns. Scale bar: 150 μm